Description
Packing : 1000 Units
HSN Code : 38220090
IMDG Identification : Not Regulated for Transport (Non-Haz)
Storage : -20 °C (Blue/Dry Ice)
Specifications
Concentration
40 U/_l
Store at -20°C
Description
RNasin is a ribonuclease inhibitor purified from human placenta. RNasin reduces the activity of RNase A-type enzymes in a variety of organisms.
Applications
Inhibition of RNA degradation in the following procedures:
-in vitro transcription
-cDNA synthesis
-in vitro translation
-isolation of mammalian cell fractions that contain mRNA-protein complex
-Separation and identification of specific ribonuclease activities
-Studies of tumor suppression
Definition of Activity Unit
One unit of the protein inhibits the activity of 5ng RNase A by 50%. Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.
